Difference between revisions of "Part:BBa K782082"

Latest revision as of 22:44, 26 September 2012

12x[NicTAL] operator_CMV promoter_2xBFP:NLS

DNA binding sites for individual TAL effectors are indicated with square brackets [ ].

Introduction

Our construct contain twelve binding sites for NicTAL12 (binding site's sequence) that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Figure 1: Schematic representation of the construct.

Characterization

Results: Specific TAL binding sites were further characterized with other reporter constructs.

BFP was obtained from Evrogen.

References

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 464
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 16
Illegal AgeI site found at 248
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1139
Illegal BsaI.rc site found at 1795
Illegal BsaI.rc site found at 2500

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K782082 short</partinfo>
+* DNA binding sites for individual TAL effectors are indicated with square brackets [ ].
@@ Line 15: / Line 16: @@
-Results: Specific TAL binding sites were further characterized with other reporter constructs.
+Results: Specific TAL [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782023 binding sites] were further characterized with other reporter constructs.
 BFP was obtained from Evrogen.
@@ Line 21: / Line 22: @@
 ==References==
-Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698
+Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.