Difference between revisions of "Part:BBa K782076"

Latest revision as of 22:34, 26 September 2012

12x[TALD] operator_CMV promoter_BFP

TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
DNA binding sites for individual TAL effectors are indicated with square brackets [ ].

Introduction

Our construct contain TALD binding sites that are cloned upstream of CMV promoter. Downstream of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Figure 1: Schematic representation of the construct.

Characterization

Results: Specific TAL binding sites were further characterized with other reporter constructs.

BFP was obtained from Evrogen.

References

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 465
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 214
Illegal AgeI site found at 446
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1141
Illegal BsaI.rc site found at 1797

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K782076 short</partinfo>
-TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
+* TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
+* DNA binding sites for individual TAL effectors are indicated with square brackets [ ].
 ==Introduction==
@@ Line 16: / Line 18: @@
-Results: Specific TAL binding sites were further characterized with other reporter constructs.
+Results: Specific TAL [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782022 binding sites] were further characterized with other reporter constructs.
 BFP was obtained from Evrogen.
 ==References==
-Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698
+Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.