Difference between revisions of "Part:BBa K801095:Design"

Latest revision as of 12:18, 3 October 2012

Naringenin - chalcone synthase (CHS) coding region

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 717
Illegal BsaI.rc site found at 1138
Illegal SapI.rc site found at 840

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/11096318/

PMID: 15932991

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, aromatic prenyltransferase

Abbreviations: CHS = naringenin-chalcone synthase

Design Notes

Related BioBrick:

Other versions: BBa_K801094: CHS + yeast consensus sequence

Related BioBricks:

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:

Protein: naringenin-chalcone synthase
Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: 2.3.1.74

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA

Organism:
Genesequence derived from Hypericum androsaemum

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AF315345.1 GenBank: Hypericum androsaemum chalcone synthase mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q9FUB7 Uniprot: Chalcone synthase]

@@ Line 12: / Line 12: @@
 <br>'''Keywords:'''
-<!--These keywords are necessary to find your part using a fulltext sarch.-->
+Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, aromatic prenyltransferase
-<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
 <br>'''Abbreviations:'''
-<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
+CHS = naringenin-chalcone synthase
-<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
 ===Design Notes===
 '''Related BioBrick:'''
-<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
-<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
+Other versions: [https://parts.igem.org/Part:BBa_K801094 BBa_K801094: CHS + yeast consensus sequence]
+Related BioBricks:
+* [https://parts.igem.org/Part:BBa_K801090 BBa_K801090: phenylalanine ammonia lyase (PAL) + yeast consensus sequence]
+* [https://parts.igem.org/Part:BBa_K801091 BBa_K801091: phenylalanine ammonia lyase (PAL) coding region]
+* [https://parts.igem.org/Part:BBa_K801092 BBa_K801092: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence]
+* [https://parts.igem.org/Part:BBa_K801093 BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region]
+* [https://parts.igem.org/Part:BBa_K801094 BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence]
+* [https://parts.igem.org/Part:BBa_K801096 BBa_K801096: Aromatic prenyltransferase (APT) coding region]
+* [https://parts.igem.org/Part:BBa_K801097 BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence]
+* [https://parts.igem.org/Part:BBa_K801098 BBa_K801098: O-methyltransferase 1 (OMT1) coding region]
 '''Cloning details:'''<br>
-<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
+Designed in RFC10/RFC25
 <!--*Mutation C889G to delete XbaI restriction site-->
 <!--*Truncation upstream/downstream compared to template, ?explanation?-->
@@ Line 33: / Line 42: @@
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
-<!--*Part was partly sequenced/Part was totally sequenced-->
+Part was totally sequenced
 '''Backbone:'''<br>
-<!--*Backbone name: pSB1C3'/?backbone_name?-->
+*Backbone name: pSB1C3
-<!--*Resistance: Amp/Cp/Kan/Tet-->
+*Resistance: Cp
-<!--*Copynumber: low/medium/high-->
+*Copynumber: high
 '''Protein coding:'''<br>
-<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
+*Protein: naringenin-chalcone synthase
 <!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 <!--*The protein encoded is posttranslationally modified by ???.-->
-<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
+*Tag: c-terminally fused Strep
-'''Enzymatic activity:'''
+'''Enzymatic activity:'''<br>
-<!--none/EC-number ?.?.?.?-->
+EC-number: 2.3.1.74
 '''Cytotoxicity:'''<br>
@@ Line 67: / Line 76: @@
 '''Source:'''<br>
 <!--*Commercial system: plasmid name, system name, company name-->
-<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
+Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA
 <!--*Preexisting BioBrick ?Bba_number?-->
 <!--*cDNA Clone: ?clone_name?, ?company_name?-->
@@ Line 76: / Line 85: @@
 '''Organism:'''<br>
-<!--*Genesequence derived from ''?organism_name?''-->
+Genesequence derived from ''Hypericum androsaemum''
 <!--*Codonoptimized for ''?organism_name?''-->
 <!--*Designed for the following Chassis: ''?organism-name?''-->
 <!--*Statement about functionality in other chassis.-->
 ===References===
@@ Line 86: / Line 94: @@
 '''Literature references:'''<br>
-<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
+[http://www.ncbi.nlm.nih.gov/pubmed/15932991 '''Pubmed''' : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]
 '''Database references:'''<br>
-<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
+[http://www.ncbi.nlm.nih.gov/nuccore/AF315345.1 '''GenBank''': Hypericum androsaemum chalcone synthase mRNA, complete cds]
 <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
-<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
+[http://www.uniprot.org/uniprot/Q9FUB7  '''Uniprot''': Chalcone synthase]
 <!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 <!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 <!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->