Difference between revisions of "Part:BBa K864404"

Latest revision as of 17:19, 20 October 2019

cjBlue Coding Device

This is an expandation of chromoprotein cjBlue BBa_K592011. It provides a turqoise color visible to the naked eye. The sequence is codon optimized for expression in E coli.

It has previously been assembled with another promotor (BBa_K592022).

Usage and Biology

This part is useful as a reporter.

iGEM12_Uppsala_University:
Left picture: Visual color expression of cjBlue with a different promotor(BBa_K592022).
Right picture: Pellet of chromoproteins eforRed BBa_K592012, RFP BBa_E1010, cjBlue BBa_K592011, aeBlue BBa_K864401, amilGFP BBa_K592010 and amilCP BBa_K592009.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K864404 short</partinfo>
-cjBlue is a blue (&#955;[maxabs]=610nm) chromoprotein from the sea anemone Cnidopus japoincus, as reported by (Chan et. al., 2006). We synthesized cjBlue codon optimized for expression in e.coli and observed a turquoise colour, wavelength approximately 5*10^2nm. This part is also submitted as a functionally active variant with J23110 and B0034
+This is an expandation of chromoprotein cjBlue [https://parts.igem.org/wiki/index.php/Part:BBa_K592011 BBa_K592011]. It provides a turqoise color visible to the naked eye. The sequence is codon optimized for expression in E coli.
+It has previously been assembled with another promotor ([https://parts.igem.org/Part:BBa_K592022 BBa_K592022]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part is useful as a reporter.
+[[Image:CjBlue_plate_small.jpg|420px]]
+[[Image:UUChromo.jpg|500px]]
+'''iGEM12_Uppsala_University:'''<br>
+''Left picture:'' Visual color expression of cjBlue with a different promotor([https://parts.igem.org/Part:BBa_K592022 BBa_K592022]). <br>
+''Right picture:'' Pellet of chromoproteins eforRed <partinfo>BBa_K592012</partinfo>, RFP <partinfo>BBa_E1010</partinfo>, cjBlue <partinfo>BBa_K592011</partinfo>, aeBlue <partinfo>BBa_K864401</partinfo>, amilGFP <partinfo>BBa_K592010</partinfo> and amilCP <partinfo>BBa_K592009</partinfo>.
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K864404 SequenceAndFeatures</partinfo>
+===Newcastle 2019 Characterisation===
 <!-- Uncomment this to enable Functional Parameter display