Difference between revisions of "Part:BBa K801012"

Latest revision as of 21:44, 26 September 2012

ADH1 yeast terminator

ADH1 terminator of S. cerevisiae

Secondary Structure

File:Mfold-K801012-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Keywords:

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:
Test digestion using XbaI+ PstI-HF

Sequencing using primer VF2 and VR

Backbone:
Backbone name: pSB1C3

Resistance: Cp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:
none

Intellectual property: PCR-Product from Vector pGADT7 AD from Clontech

Corresponding part author/authors:

Source

Source:
Vector pGADT7 AD, Company: Clonetech

Organism:
Saccharomyces cerevisiae

References

Literature references:
Chien, C. T., Bartel, P. L., Sternglanz, R. & Fields, S. (1991) Proc. Natl. Acad. Sci. USA 88:9578–9582.

Database references:

@@ Line 35: / Line 35: @@
 <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
-'''Cloning details:'''<br>
+'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25/RFC25
 <!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 <!--*Mutation C889G to delete XbaI restriction site-->
 <!--*Truncation upstream/downstream compared to template, ?explanation?-->
-'''Quality control measures:'''<br>
+'''Quality control measures:'''<br> Test digestion using XbaI+ PstI-HF
+*Sequencing using primer VF2 and VR
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 <!--*Part was partly sequenced/Part was totally sequenced-->
-'''Backbone:'''<br>
+'''Backbone:'''<br> Backbone name: pSB1C3
+* Resistance: Cp
 <!--*Backbone name: pSB1C3'/?backbone_name?-->
 <!--*Resistance: Amp/Cp/Kan/Tet-->
@@ Line 62: / Line 64: @@
 <!--none/not known/cytotoxic for ''organism name''-->
-'''Safety notes:'''<br>
+'''Safety notes:'''<br> none
 <!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 <!--Known and anticipated security issues: none/other.-->
-'''Intellectual property:'''
+'''Intellectual property:''' PCR-Product from Vector pGADT7 AD from Clontech
 <!--Information on patent situation.-->
 <!--Intellectual property claims made by the authors.-->
@@ Line 75: / Line 77: @@
 ===Source===
-'''Source:'''<br>
+'''Source:'''<br> Vector pGADT7 AD, Company: Clonetech
 <!--*Commercial system: plasmid name, system name, company name-->
 <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
@@ Line 85: / Line 87: @@
 <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
-'''Organism:'''<br>
+'''Organism:'''<br> ''Saccharomyces cerevisiae''
 <!--*Genesequence derived from ''?organism_name?''-->
 <!--*Codonoptimized for ''?organism_name?''-->
 <!--*Designed for the following Chassis: ''?organism-name?''-->
 <!--*Statement about functionality in other chassis.-->
 ===References===
 <!-- Here you find templates to insert references to literature and different databases-->
-'''Literature references:'''<br>
+'''Literature references:'''<br> Chien, C. T., Bartel, P. L., Sternglanz, R. & Fields, S. (1991) Proc. Natl. Acad. Sci. USA 88:9578–9582.
 <!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->