Difference between revisions of "Part:BBa K801010:Design"

Latest revision as of 22:02, 26 September 2012

TEF2 constitutive yeast promoter

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 137
1000
COMPATIBLE WITH RFC[1000]

Extracted from genomic DNA of S. cerevisiae using PCR.

Keywords:

Abbreviations:

Design Notes

Related BioBrick:BBa_K165037

Quality control measures:
Test digestion using XbaI+ PstI-HF, SpeI-HF+ PstI-HF

Backbone:
Backbone name: pSB1C3, Resistance: Cp

Protein coding:

Enzymatic activity:

Cytotoxicity:
none

Safety notes:
none

Intellectual property:

Corresponding part author/authors:

Source

Source:
PCR from genomic DNA of Saccharomyces cerevisiae

Forward Primer: VF2
Reverse Primer: VR

Organism:
Developed for Saccharomyces cerevisiae

References

Literature references:

Schirmaier, F.; Philippsen, P. (1984): Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae. In: EMBO J. 3 (13), S. 3311–3315.
Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.

Database references:

http://ypa.ee.ncku.edu.tw/index.html

@@ Line 21: / Line 21: @@
 ===Design Notes===
-'''Related BioBrick:'''
+'''Related BioBrick:'''[https://parts.igem.org/wiki/index.php?title=Part:BBaBBa_K165037: BBa_K165037]
 <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
-'''Cloning details:'''<br>
+'''Cloning details:'''<br> Designed in RFC10/RFC23
 <!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 <!--*Mutation C889G to delete XbaI restriction site-->
 <!--*Truncation upstream/downstream compared to template, ?explanation?-->
-'''Quality control measures:'''<br>
+'''Quality control measures:'''<br> Test digestion using XbaI+ PstI-HF, SpeI-HF+ PstI-HF
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 <!--*Part was partly sequenced/Part was totally sequenced-->
-'''Backbone:'''<br>
+'''Backbone:'''<br> Backbone name: pSB1C3, Resistance: Cp
 <!--*Backbone name: pSB1C3'/?backbone_name?-->
 <!--*Resistance: Amp/Cp/Kan/Tet-->
@@ Line 49: / Line 49: @@
 <!--none/EC-number ?.?.?.?-->
-'''Cytotoxicity:'''<br>
+'''Cytotoxicity:'''<br> none
 <!--none/not known/cytotoxic for ''organism name''-->
-'''Safety notes:'''<br>
+'''Safety notes:'''<br> none
 <!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 <!--Known and anticipated security issues: none/other.-->
@@ Line 65: / Line 65: @@
 ===Source===
-'''Source:'''<br>
+'''Source:'''<br> PCR from genomic DNA of ''Saccharomyces cerevisiae''
 <!--*Commercial system: plasmid name, system name, company name-->
 <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
@@ Line 71: / Line 71: @@
 <!--*cDNA Clone: ?clone_name?, ?company_name?-->
 <!--*Synthesized by ?company_name?.-->
+* Forward Primer: VF2
+* Reverse Primer: VR
 <!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
-'''Organism:'''<br>
+'''Organism:'''<br> Developed for ''Saccharomyces cerevisiae''
 <!--*Genesequence derived from ''?organism_name?''-->
 <!--*Codonoptimized for ''?organism_name?''-->
 <!--*Designed for the following Chassis: ''?organism-name?''-->
 <!--*Statement about functionality in other chassis.-->
 ===References===
@@ Line 86: / Line 86: @@
 '''Literature references:'''<br>
+*Schirmaier, F.; Philippsen, P. (1984): Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae. In: EMBO J. 3 (13), S. 3311–3315.
+*Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.
 <!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 '''Database references:'''<br>
+*http://ypa.ee.ncku.edu.tw/index.html
 <!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
 <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->