Difference between revisions of "Part:BBa K801001:Design"

Latest revision as of 15:20, 3 October 2012

pTUM101 yeast shuttle vector with pTEF1 promoter

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 36
Illegal suffix found at 506
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal NheI site found at 3771
Illegal SpeI site found at 507
Illegal PstI site found at 521
Illegal NotI site found at 42
Illegal NotI site found at 514
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 36
Illegal suffix found at 507
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 36
Plasmid lacks a suffix.
Illegal XbaI site found at 51
Illegal SpeI site found at 507
Illegal PstI site found at 521
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 261
Illegal BsaI.rc site found at 1928
Illegal BsaI.rc site found at 2874
Illegal SapI site found at 845
Illegal SapI site found at 3026

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

Keywords: shuttle vector yeast, pTEF1

Abbreviations:

Design Notes

Related BioBrick:

Other versions:

Quality control measures:
Test digestion using SpeI+ PstI

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Commercial system: pYES2, Invitrogen

Organism:
Designed for the following Chassis:Saccharomyces cerevisiae, Escherichia coli

References

Literature references:

Database references:

@@ Line 10: / Line 10: @@
 <br>'''Keywords:'''
 <!--These keywords are necessary to find your part using a fulltext sarch.-->
-<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
+shuttle vector yeast, pTEF1
 <br>'''Abbreviations:'''
@@ Line 18: / Line 18: @@
 ===Design Notes===
 '''Related BioBrick:'''
+Other versions:
+* [https://parts.igem.org/Part:BBa_K801000 BBa_K801000: pTUM100 yeast shuttle vector based on pYES2]
+* [https://parts.igem.org/Part:BBa_K801002 BBa_K801002: pTUM102 yeast shuttle vector with pTEF2 promoter]
+* [https://parts.igem.org/Part:BBa_K801003 BBa_K801003: pTUM103 yeast shuttle vector with pADH1 promoter]
+* [https://parts.igem.org/Part:BBa_K801004 BBa_K801004: pTUM104 yeast shuttle vector with GAL1 promoter]
 <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
-'''Cloning details:'''<br>
+'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25
 <!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 <!--*Mutation C889G to delete XbaI restriction site-->
 <!--*Truncation upstream/downstream compared to template, ?explanation?-->
-'''Quality control measures:'''<br>
+'''Quality control measures:'''<br> Test digestion using SpeI+ PstI
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 <!--*Part was partly sequenced/Part was totally sequenced-->
-'''Backbone:'''<br>
+'''Backbone:'''<br> Resistance: Amp
 <!--*Backbone name: pSB1C3'/?backbone_name?-->
 <!--*Resistance: Amp/Cp/Kan/Tet-->
@@ Line 62: / Line 68: @@
 ===Source===
-'''Source:'''<br>
+'''Source:'''<br> Commercial system: pYES2, Invitrogen
 <!--*Commercial system: plasmid name, system name, company name-->
 <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
@@ Line 72: / Line 78: @@
 <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
-'''Organism:'''<br>
+'''Organism:'''<br> Designed for the following Chassis:''Saccharomyces cerevisiae'', ''Escherichia coli''
 <!--*Genesequence derived from ''?organism_name?''-->
 <!--*Codonoptimized for ''?organism_name?''-->
 <!--*Designed for the following Chassis: ''?organism-name?''-->
 <!--*Statement about functionality in other chassis.-->
 ===References===