Difference between revisions of "Part:BBa K782006"
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| − | TALB label represents TAL effector 1297 from zebrafish experiments (Sander et al., 2011). | + | *TALB label represents TAL effector 1297 from zebrafish experiments (Sander et al., 2011). |
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==Introduction== | ==Introduction== | ||
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TAL effectors (TALEs) are bacterial plant pathogen transcription factors, that bind to DNA by specifically recognizing one base pair with a single tandem repeat in their DNA-binding domain. A tandem TALE repeat contains 33 to 35 amino acids, where the 12th and 13th amino acid, called a “repeat variable diresidue” (RVD), are responsible for specific interactions with the corresponding base pair (Scholze and Boch, 2011). | TAL effectors (TALEs) are bacterial plant pathogen transcription factors, that bind to DNA by specifically recognizing one base pair with a single tandem repeat in their DNA-binding domain. A tandem TALE repeat contains 33 to 35 amino acids, where the 12th and 13th amino acid, called a “repeat variable diresidue” (RVD), are responsible for specific interactions with the corresponding base pair (Scholze and Boch, 2011). | ||
| − | [[Image:Svn_12_TALB.png]] | + | [[Image:Svn_12_TALB--.png]] |
Figure 1: Schematic representation of the construct. | Figure 1: Schematic representation of the construct. | ||
Single binding sequence for TALB: tCTTCCGTTTCCACATCT | Single binding sequence for TALB: tCTTCCGTTTCCACATCT | ||
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==Characterization== | ==Characterization== | ||
| − | TAL effector was | + | TAL effector was was provided by host lab and cloned into a biobrick vector. This construct was later used for designing TAL-based activator and repressor by adding [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782013 VP16] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782010 KRAB] domain. |
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==References== | ==References== | ||
Latest revision as of 12:51, 26 September 2012
TALB:NLS DNA binding domain
- TALB label represents TAL effector 1297 from zebrafish experiments (Sander et al., 2011).
Introduction
TAL effectors (TALEs) are bacterial plant pathogen transcription factors, that bind to DNA by specifically recognizing one base pair with a single tandem repeat in their DNA-binding domain. A tandem TALE repeat contains 33 to 35 amino acids, where the 12th and 13th amino acid, called a “repeat variable diresidue” (RVD), are responsible for specific interactions with the corresponding base pair (Scholze and Boch, 2011).
Figure 1: Schematic representation of the construct.
Single binding sequence for TALB: tCTTCCGTTTCCACATCT
Characterization
TAL effector was was provided by host lab and cloned into a biobrick vector. This construct was later used for designing TAL-based activator and repressor by adding VP16 and KRAB domain.
References
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698
Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2439
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]