Difference between revisions of "Part:BBa K741004:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Protein CI is expressed to activated the promoter pRM. Thus different concentration of CI lead to different strength of green fluorescence. | + | Protein CI is expressed to activated the promoter pRM. Thus different concentration of CI lead to different strength of green fluorescence. We use this part to test the character of modified pRM (BBa_I12007) being activated by CI. |
===Source=== | ===Source=== |
Latest revision as of 00:36, 3 October 2012
plac-RBS-cI-T-pRM-RBS-GFP-T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1898
Design Notes
Protein CI is expressed to activated the promoter pRM. Thus different concentration of CI lead to different strength of green fluorescence. We use this part to test the character of modified pRM (BBa_I12007) being activated by CI.
Source
BBa_R0010 BBa_P0451 BBa_I13504 BBa_I12007