Difference between revisions of "Part:BBa K823041"

Latest revision as of 05:53, 1 November 2014

PydfG promoter regulated by ecf41

P_ydfG is a promotor from B. lincheniformes and is regulated by the alternative σ factor ecf41_{Bli aa 1-204}.

Our team used this part in the combination with P_spoIVB/P_sspK,ecf41_{Bli aa 1-204} and MazF in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch Suicide switch].

σ G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.

This Platereader measurment is the first and not reproduced to this point!

This Platereader assay was done with the Bacillus subtilis strain W168 containing thrC::P_spoIVB-ecf41_{Bli aa 1-204} (through transformation with pSBBs4S-P_spoIVB-ecf41_{Bli aa 1-204}) and sacA::P_ydfG-luxABCDE (through transformation with pSBBs3C-luxABCDE-P_ydfG). That we get a signal shows that this is active together with ecf41_{Bli aa 1-204}.

Fig. 1. Platereader measurement of ECF41 delay

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K823041 short</partinfo>
-PydfG is a promotor from B. lincheniformes and is regulated by the alternative sigma factor ecf41
+P<sub>''ydfG''</sub> is a promotor from ''B. lincheniformes'' and is regulated by the alternative σ factor ecf41<sub>Bli aa  1-204</sub>.
-This is an alternative sigma factor from B. lincheniformes regulating e.g. PydfG. It is the short version lacking the regulation domain and therefore constitutively on.
 [[Image:SuicideSwitchgrafik.jpg|600px]]
-Our team used this part in the combination with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823048 PspoIVB]/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K823042 PsspK],[https://parts.igem.org/Part:BBa_K823043 ECF41] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823044 MazF] in the <b>Suicide</b> switch.
+Our team used this part in the combination with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823048 P<sub>''spoIVB''</sub>]/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K823042 P<sub>''sspK''</sub>],[https://parts.igem.org/Part:BBa_K823043 ecf41<sub>Bli aa  1-204</sub>] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823044 MazF] in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch <b>Suicide</b> switch].
-Sigma G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.
+σ G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.
 <b>This Platereader measurment is the first and not reproduced to this point!</b>
 [[Image:PlatereaderPspoIVBecf41PydfG.jpg|600px]]
-This Platereader assay was done with the ''Bacillus subtilis'' strain W168 containing thrC::PspoIVB-ECF41 (through transformation with pSBBs4S-PspoIVB-ECF41) and sacA::pydfG-luxABCDE (through transformation with pSBBs3C-luxABCDE-PydfG). That we get a signal shows that this is active together with [https://parts.igem.org/Part:BBa_K823043 ECF41].
+This Platereader assay was done with the ''Bacillus subtilis'' strain W168 containing ''thrC''::P<sub>''spoIVB''</sub>-''ecf41<sub>Bli aa  1-204</sub>'' (through transformation with pSBBs4S-P<sub>''spoIVB''</sub>-''ecf41<sub>Bli aa  1-204</sub>'') and ''sacA''::P<sub>''ydfG''</sub>-''luxABCDE'' (through transformation with pSBBs3C-''luxABCDE''-P<sub>''ydfG''</sub>). That we get a signal shows that this is active together with [https://parts.igem.org/Part:BBa_K823043 ecf41<sub>Bli aa  1-204</sub>].
+[[File:delay prezi.png|thumb|600px|center|Fig. 1. Platereader measurement of ECF41 delay]]
-<!-- Add more about the biology of this part here
-===Usage and Biology===
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