Difference between revisions of "Part:BBa K819017:Design"

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===Design Notes===
 
===Design Notes===
−
The original SulA with the binding site of CTGT(N)<sub>8</sub>ACAG is mutated to CCGT(N)<sub>8</sub>ACGG, which is the 408 form.<br />The LexA 408 protein has corresponding mutations of PA40, NS41 and AS42.
+
The original SulA with the binding site of CTGT(N)<sub>8</sub>ACAG is mutated to CCGT(N)<sub>8</sub>ACGG, which is the 408 form.<br />The LexA408 protein has corresponding mutations of PA40, NS41 and AS42.
  
  
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===Source===
 
===Source===
  
−
SulA promoter sequence was from [http://regulondb.ccg.unam.mx/gene?organism=ECK12&term=ECK120000973&format=jsp&type=gene RegulonDB]<br />The promoter was synthesized by PCR<br />
+
SulA promoter sequence was from [http://regulondb.ccg.unam.mx/gene?organism=ECK12&term=ECK120000973&format=jsp&type=gene RegulonDB]<br />The mutant promoter was synthesized by PCR and site directed mutagenesis<br />
  
 
===References===
 
===References===
 +
1. Cole, S.T.(1983) Charaeterisation of the Promoter
for the LexA Regulated sulA Gene of Escherichia coli. Mol. Gen. Genet., 189: 400: 404 <br />
 +
2. Zhang, A.P.P., Pigli, Y.Z & Rice, P.A.(2010) Structure of the LexA–DNA complex and implications for SOS box measurement.Nature, 466: 883: 886 <br />
 +
3. Butalaa, M., Zgur-Bertokb, D., and Busby, S. J. W.(2009) The bacterial LexA transcriptional repressor. Cell. Mol. Life Sci., 66: 82: 93<br />

Latest revision as of 09:41, 26 September 2012

Luminesensor repressible SulA408 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original SulA with the binding site of CTGT(N)8ACAG is mutated to CCGT(N)8ACGG, which is the 408 form.
The LexA408 protein has corresponding mutations of PA40, NS41 and AS42.


Source

SulA promoter sequence was from [http://regulondb.ccg.unam.mx/gene?organism=ECK12&term=ECK120000973&format=jsp&type=gene RegulonDB]
The mutant promoter was synthesized by PCR and site directed mutagenesis

References

1. Cole, S.T.(1983) Charaeterisation of the Promoter
for the LexA Regulated sulA Gene of Escherichia coli. Mol. Gen. Genet., 189: 400: 404
2. Zhang, A.P.P., Pigli, Y.Z & Rice, P.A.(2010) Structure of the LexA–DNA complex and implications for SOS box measurement.Nature, 466: 883: 886
3. Butalaa, M., Zgur-Bertokb, D., and Busby, S. J. W.(2009) The bacterial LexA transcriptional repressor. Cell. Mol. Life Sci., 66: 82: 93