Difference between revisions of "Part:BBa K792011"

Latest revision as of 20:37, 27 October 2012

Yeast exportable His-rich peptide w/enhanced import (2)

This composite device produces and exports a Histidine rich peptide, that is import enhanced (thanks to a trojan peptide). This device is intended to be used with Yeast chassis, so it is composed by subparts designed also to be use in yeast.

The high level structure of the device is:

BamHI restriction site (introduced to facilitate cloning into yeast expression vectors (pEG202, pCM180 series) - this is not essential for the device function)
Kozak consensus sequence for initiation of translation
Signal peptide that targets the product of the gene for secretion
Trojan peptide, to increase internalization in target cell (Polyarginine)
Payload: this is the exported Histidine rich domain of the protein (a custom designed peptide that we called PolyHb)

The device's sequence has inherited HindIII restriction site from the parts it's made of.

Important note:

this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from BB assembly standard.

See also:	Another His-rich peptide export and enhanced import composite device Part:BBa_K792009.
	Similar design Trp-rich peptide export and enhanced import composite device Part:BBa_K792012.

This device contains BBa_K792004, a trojan peptide. Extreme your safety measures when dealing with strains containing this part: avoid direct contact with them or conditioned medium and discard them as pathogenic residues.

The device was originally designed and built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details. Also feel free to contact us for any question regarding this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 The high level structure of the device is:
-* '''''BamHI'''''restriction site
+* '''''BamHI''''' restriction site ''(introduced to facilitate cloning into yeast expression vectors (pEG202, pCM180 series) - '''this is not essential''' for the device function)''
 * '''''Kozak''''' consensus sequence for initiation of translation
 * '''''Signal''''' peptide that targets the product of the gene for secretion
@@ Line 15: / Line 15: @@
-The device's sequence has inherited some useful '''restriction sites''' (''BamHI'' & ''HindIII'') from the parts it's made of.
+The device's sequence has inherited ''HindIII'' restriction site from the parts it's made of.
-'''Important note''': this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from ''BB assembly standard''.
+{| style="width:100%"
+|-
+| style="background: red; width=11%" | '''Important note: '''
+| style="background: #D8D8D8" | this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from ''BB assembly standard''.
+|}
+{| style="width:100%"
+|-
+| style="background: yellow; width:11%" rowspan="2" | '''See also: '''
+| style="background: #D8D8D8" | Another '''''His-rich peptide export and enhanced import''''' composite device [[Part:BBa_K792009]].
+|-
+| style="background: #D8D8D8" | Similar design '''''Trp-rich peptide export and enhanced import''''' composite device [[Part:BBa_K792012]].
+|}
+{| style="width:100%; background: #FFBF00"
+|-
+| [[Image:BsAs2012-Biohazard.png | 70px]]
+| This device contains <partinfo>K792004</partinfo>, a trojan peptide. Extreme your safety measures when dealing with strains containing this part: '''avoid direct contact with them or conditioned medium and discard them as pathogenic residues.'''
+|}
+{| style="width:100%; background: #CEE3F6"
+|-
+|The device was originally designed and built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a ''cross-feeding'' regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details. Also feel free to contact us for any question regarding this part.
+|[[Image:Igem.bsas.png | 100px]]
+|}
 <!-- Add more about the biology of this part here