Difference between revisions of "Part:BBa K750009:Design"

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===Design Notes===
 
===Design Notes===
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editing
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Lasr year, we use IPTG to induct the production of LuxI protein and LuxR protein. While this year we hope that there are an obvious process from dark to light. Because of the strong basal expression of promoter LuxPR(BBa_R0062), we have to change the promoter LuxPR(BBa_R0062) to Pbad(BBa_K206000).
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+
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===Source===
 
===Source===
  
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We built this part by biobricks from the DNA distribution kit plates 2012.
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We built this part by biobricks from the DNA distribution kit plates 2012. The inspiration of design this part comes from further research of the iccdB project we designed last year.
  
 
===References===
 
===References===
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1. W.Claiborne Fuqua, S.C.W., E. Peter Greenberg, Quorum Sensing in Bacteria: the LuxR-LuxI Family of Cell Density-Responsive Transcriptional Regulatorst. JOURNAL OF BACTERIOLOGY, 1994. 176(2): p. 269-275.<br>
+
[1]W.Claiborne Fuqua, S.C.W., E. Peter Greenberg, Quorum Sensing in Bacteria: the LuxR-LuxI Family of Cell Density-Responsive Transcriptional Regulatorst. JOURNAL OF BACTERIOLOGY, 1994. 176(2): p. 269-275.<br><br>
−
2. You, L., et al., Programmed population control by cell-cell communication and regulated killing. Nature, 2004. 428(6985): p. 868-71.<br>
+
[2]You, L., et al., Programmed population control by cell-cell communication and regulated killing. Nature, 2004. 428(6985): p. 868-71.<br><br>
−
3. Alon, U., Network motifs: theory and experimental approaches. Nat Rev Genet, 2007. 8(6): p. 450-61.<br>
+
[3]Alon, U., Network motifs: theory and experimental approaches. Nat Rev Genet, 2007. 8(6): p. 450-61.<br><br>
−
4. Mangan, S., et al., The incoherent feed-forward loop accelerates the response-time of the gal system of Escherichia coli. J Mol Biol, 2006. 356(5): p. 1073-81.<br>
+
[4]Mangan, S., et al., The incoherent feed-forward loop accelerates the response-time of the gal system of Escherichia coli. J Mol Biol, 2006. 356(5): p. 1073-81.<br><br>
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5. Camas, F.M., J. Blazquez, and J.F. Poyatos, Autogenous and nonautogenous control of response in a genetic network. Proc Natl Acad Sci U S A, 2006. 103(34): p. 12718-23.<br>
+
[5]Camas, F.M., J. Blazquez, and J.F. Poyatos, Autogenous and nonautogenous control of response in a genetic network. Proc Natl Acad Sci U S A, 2006. 103(34): p. 12718-23.<br><br>
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6．The project iccdB of team XMU-China in iGEM 2011: http://2011.igem.org/Team:XMU-China<br>
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[6]http://2011.igem.org/Team:XMU-China<br><br>

Latest revision as of 14:12, 25 September 2012

TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1069
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 793
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2024
    Illegal BsaI.rc site found at 2751


Design Notes

Lasr year, we use IPTG to induct the production of LuxI protein and LuxR protein. While this year we hope that there are an obvious process from dark to light. Because of the strong basal expression of promoter LuxPR(BBa_R0062), we have to change the promoter LuxPR(BBa_R0062) to Pbad(BBa_K206000).

Source

We built this part by biobricks from the DNA distribution kit plates 2012. The inspiration of design this part comes from further research of the iccdB project we designed last year.

References

[1]W.Claiborne Fuqua, S.C.W., E. Peter Greenberg, Quorum Sensing in Bacteria: the LuxR-LuxI Family of Cell Density-Responsive Transcriptional Regulatorst. JOURNAL OF BACTERIOLOGY, 1994. 176(2): p. 269-275.

[2]You, L., et al., Programmed population control by cell-cell communication and regulated killing. Nature, 2004. 428(6985): p. 868-71.

[3]Alon, U., Network motifs: theory and experimental approaches. Nat Rev Genet, 2007. 8(6): p. 450-61.

[4]Mangan, S., et al., The incoherent feed-forward loop accelerates the response-time of the gal system of Escherichia coli. J Mol Biol, 2006. 356(5): p. 1073-81.

[5]Camas, F.M., J. Blazquez, and J.F. Poyatos, Autogenous and nonautogenous control of response in a genetic network. Proc Natl Acad Sci U S A, 2006. 103(34): p. 12718-23.

[6]http://2011.igem.org/Team:XMU-China