Difference between revisions of "Part:BBa K883701:Design"
Lisamsonne (Talk | contribs) (→Design Notes) |
Lisamsonne (Talk | contribs) |
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| Line 17: | Line 17: | ||
|translated | |translated | ||
|- | |- | ||
| − | | | + | |K-coil (counterpart) |
|aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag | |aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag | ||
|KIAALKEKIAALKEKIAALKE | |KIAALKEKIAALKEKIAALKE | ||
|- | |- | ||
| − | | | + | |E-coil (present in the brick) |
|gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa | |gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa | ||
|EIAALEKEIAALEKEIAALEK | |EIAALEKEIAALEKEIAALEK | ||
Latest revision as of 15:23, 25 September 2012
GFP with a His-tag fused to a coiled coil
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 710
Design Notes
To be able to use the device, the counterpart of the E-coil needs to be fused to the protein that needs to be detected.
| name | sequence | translated |
| K-coil (counterpart) | aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag | KIAALKEKIAALKEKIAALKE |
| E-coil (present in the brick) | gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa | EIAALEKEIAALEKEIAALEK |
Source
The biobrick BBa_I13522 was used as a template for the mutations