Difference between revisions of "Part:BBa K784043"
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The T7 RNAP part contains a weak ribosome binding site and 'GTG' start codon . | The T7 RNAP part contains a weak ribosome binding site and 'GTG' start codon . | ||
The native T7 RNAP differs from the mutant one by its toxicity for the host cell- the mutant have a low toxicity than the native one (according to Voight's article), this treat for low toxicity was accrued by a Spontaneous mutation, within the active site, during the cloning and doesn’t reduce the RNAP activity. | The native T7 RNAP differs from the mutant one by its toxicity for the host cell- the mutant have a low toxicity than the native one (according to Voight's article), this treat for low toxicity was accrued by a Spontaneous mutation, within the active site, during the cloning and doesn’t reduce the RNAP activity. | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 21:11, 25 September 2012
Mutant T7 RNAP - T7*
This part includes an engineered T7 RNAP with a RBS, the RNAP recognizes specifically a suitable promoter (pT7). The mutant T7 RNAP was donated us by Chris Voight. The T7 RNAP part contains a weak ribosome binding site and 'GTG' start codon . The native T7 RNAP differs from the mutant one by its toxicity for the host cell- the mutant have a low toxicity than the native one (according to Voight's article), this treat for low toxicity was accrued by a Spontaneous mutation, within the active site, during the cloning and doesn’t reduce the RNAP activity.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]