Difference between revisions of "Part:BBa K823029"
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[[Image:LMU-Munich-MKate Pellet.JPG|<p align="justify">'''''mKate2'' fused to the terminator B0014 under the control of the Anderson promoter J23101 (up), P<sub>''liaI''</sub> (middle) and [https://parts.igem.org/Part:BBa_K823002 P<sub>''lepA''</sub>] (down) in [https://parts.igem.org/Part:BBa_K8230023 pSB<sub>''Bs''</sub>1C]'''. Pellets are ''Escherichia coli'' cells which contain the plasmid with the right insert.</p>|thumb|300px|right]]mKate2 is a far-red fluorescent protein that is monomeric and extremely photostable. It is in Freiburg standard and has a RBS included with the correct spacing. | [[Image:LMU-Munich-MKate Pellet.JPG|<p align="justify">'''''mKate2'' fused to the terminator B0014 under the control of the Anderson promoter J23101 (up), P<sub>''liaI''</sub> (middle) and [https://parts.igem.org/Part:BBa_K823002 P<sub>''lepA''</sub>] (down) in [https://parts.igem.org/Part:BBa_K8230023 pSB<sub>''Bs''</sub>1C]'''. Pellets are ''Escherichia coli'' cells which contain the plasmid with the right insert.</p>|thumb|300px|right]]mKate2 is a far-red fluorescent protein that is monomeric and extremely photostable. It is in Freiburg standard and has a RBS included with the correct spacing. | ||
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+ | Find out more about the design of our [https://static.igem.org/mediawiki/parts/b/b9/LMU-Munich_2012_Our_Freiburg_standard_FusionPrefix.pdf prefix with ribosome binding site]. | ||
prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC | prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC | ||
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− | <p align="justify">We cloned this reporter in front of the terminator B0014. For the evaluation this reporter was successfully combined with the promoters [https://parts.igem.org/Part:BBa_K823001 P<sub>''liaI''</sub>], P<sub>''lepA''</sub> and the Anderson promoter [https://parts.igem.org/Part:BBa_K823005 J23101] in the empty ''Bacillus'' vector [https://parts.igem.org/Part:BBa_K823023 pSB<sub>''Bs''</sub>1C] from our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox]. At the moment we have the right clones of ''B. subtilis'' with the integrated construct. Unfortunately we have no equipment to measure this reporter. Neither our plate reader nor the fluorescent microscope | + | <p align="justify">We cloned this reporter in front of the terminator B0014. For the evaluation this reporter was successfully combined with the promoters [https://parts.igem.org/Part:BBa_K823001 P<sub>''liaI''</sub>], P<sub>''lepA''</sub> and the Anderson promoter [https://parts.igem.org/Part:BBa_K823005 J23101] in the empty ''Bacillus'' vector [https://parts.igem.org/Part:BBa_K823023 pSB<sub>''Bs''</sub>1C] from our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox]. At the moment we have the right clones of ''B. subtilis'' with the integrated construct. Unfortunately we have no equipment to measure this reporter. Neither our plate reader nor the fluorescent microscope have the required filters.</p> |
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Latest revision as of 08:54, 26 September 2012
mKate2, a red monomeric fluorescent protein, B. subtilis optimized
mKate2 is a far-red fluorescent protein that is monomeric and extremely photostable. It is in Freiburg standard and has a RBS included with the correct spacing.Find out more about the design of our prefix with ribosome binding site.
prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
Excitation maximum: 588 nm
Emission maximum: 633 nm
We cloned this reporter in front of the terminator B0014. For the evaluation this reporter was successfully combined with the promoters PliaI, PlepA and the Anderson promoter J23101 in the empty Bacillus vector pSBBs1C from our [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox]. At the moment we have the right clones of B. subtilis with the integrated construct. Unfortunately we have no equipment to measure this reporter. Neither our plate reader nor the fluorescent microscope have the required filters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
- 1000COMPATIBLE WITH RFC[1000]