Difference between revisions of "Part:BBa K784038"
 
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<partinfo>BBa_K784038 short</partinfo>
 
<partinfo>BBa_K784038 short</partinfo>
  
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This part has the K1F RNA Polymerase gene. This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells. This part was recieved from Chris Voight lab (see sources). In order to achieve reduced levels of toxicity Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and GTG start codon.
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This part includes T7*(K1F) RNA Polymerase gene. This is orthogonal polymerases, based on T7 wt RNAP, that recognizes specifically a suitable promoter- pK1F. The T7*(K1F) RNA Polymerase was donated us by Chris Voight lab (see source).
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The T7*(K1F) RNA Polymerase is less toxic to host cells, than the T7 wt. In order to achieve reduced levels of toxicity, Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and ATG start codon. The specificity of the T7*(K1F) to pK1F was achieved by engineering a DNA binding domain within the RNA polymerase.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 12:23, 26 September 2012

T7*(K1F) RNA polymerase

This part includes T7*(K1F) RNA Polymerase gene. This is orthogonal polymerases, based on T7 wt RNAP, that recognizes specifically a suitable promoter- pK1F. The T7*(K1F) RNA Polymerase was donated us by Chris Voight lab (see source). The T7*(K1F) RNA Polymerase is less toxic to host cells, than the T7 wt. In order to achieve reduced levels of toxicity, Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and ATG start codon. The specificity of the T7*(K1F) to pK1F was achieved by engineering a DNA binding domain within the RNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]