Difference between revisions of "Part:BBa K737045"
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The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42. It enables strong competition with the galk::GFP to interact with spot42 which shows great potential for constructing sRNA-mediated circuit. | The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42. It enables strong competition with the galk::GFP to interact with spot42 which shows great potential for constructing sRNA-mediated circuit. | ||
| − | This device | + | This device means to test feasibility of N/P comparator,replacing Nitrate,Phosphate inputs by IPTG,aTc. |
Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one. | Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one. | ||
Spot42 sRNA is under control of aTc inducible device,the buffer RNA YtfJ is under control of IPTG inducible device. | Spot42 sRNA is under control of aTc inducible device,the buffer RNA YtfJ is under control of IPTG inducible device. | ||
| − | https://static.igem.org/mediawiki/parts/ | + | https://static.igem.org/mediawiki/parts/9/90/2-1.jpg |
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| + | https://static.igem.org/mediawiki/parts/e/ec/Gmh2.PNG | ||
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| + | The pairing hybridization energy is -12.2 kcal/mol. Although 7 bases are involved in the pairing, microarray analysis indicates that it can give more than 5-fold repression rate and only±0.2 experimental error. | ||
===Experimental Data=== | ===Experimental Data=== | ||
| − | https://static.igem.org/mediawiki/parts/ | + | |
| + | https://static.igem.org/mediawiki/parts/4/4d/YTFJ.3.png | ||
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| + | Figure 1 ODE simulation of comparator | ||
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| + | https://static.igem.org/mediawiki/parts/8/8b/Ytfj_control.png | ||
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| + | Figure 2 Experimental result <!-- --> Figure 3 Expectation effect by ODE simulation | ||
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| + | In comparison,we can see YtfJ in accordance with our expectation. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 16:57, 26 September 2012
YtfJ_Comparator
The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42. It enables strong competition with the galk::GFP to interact with spot42 which shows great potential for constructing sRNA-mediated circuit. This device means to test feasibility of N/P comparator,replacing Nitrate,Phosphate inputs by IPTG,aTc. Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one.
Spot42 sRNA is under control of aTc inducible device,the buffer RNA YtfJ is under control of IPTG inducible device.
The pairing hybridization energy is -12.2 kcal/mol. Although 7 bases are involved in the pairing, microarray analysis indicates that it can give more than 5-fold repression rate and only±0.2 experimental error.
Experimental Data
Figure 1 ODE simulation of comparator
Figure 2 Experimental result Figure 3 Expectation effect by ODE simulation
In comparison,we can see YtfJ in accordance with our expectation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1231
Illegal NheI site found at 1254
Illegal NheI site found at 2769
Illegal NheI site found at 2792 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2432
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1181
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3581