Difference between revisions of "Part:BBa K886001:Design"
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===References=== | ===References=== | ||
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+ | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/ | ||
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+ | http://2012.igem.org/Team:USP-UNESP-Brazil/Plasmid_Plug_n_Play/Introduction |
Latest revision as of 22:41, 24 September 2012
Recombination Device composed by lox71-Cre recombinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 396
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used the lox71 submitted by our group BBa_K886000, which corrected the parts BBa_I718017 and BBa_K537020. Those parts are wrong when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/
Source
We synthesized the lox71 site, then we assembled it upstream the Cre recombinase gene(BBa_J61047).
References
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/
http://2012.igem.org/Team:USP-UNESP-Brazil/Plasmid_Plug_n_Play/Introduction