Difference between revisions of "Part:BBa K784035"

Latest revision as of 12:30, 26 September 2012

T7*(N4) RNA polymerase

This part includes T7*(N4) RNA Polymerase gene. This is orthogonal polymerases, based on T7 wt RNAP, that recognizes specifically a suitable promoter- pK1F. The T7*(N4) RNA Polymerase was donated us by Chris Voight lab (see source). The T7*(N4) RNA Polymerase is less toxic to host cells, than the T7 wt. In order to achieve reduced levels of toxicity, Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and ATG start codon. The specificity of the T7*(N4) to pK1F was achieved by engineering a DNA binding domain within the RNA polymerase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K784035 short</partinfo>
-This part has the N4 RNA Polymerase gene. This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells.
+This part includes T7*(N4) RNA Polymerase gene. This is orthogonal polymerases, based on T7 wt RNAP, that recognizes specifically a suitable promoter- pK1F. The T7*(N4) RNA Polymerase was donated us by Chris Voight lab (see source). The T7*(N4) RNA Polymerase is less toxic to host cells, than the T7 wt. In order to achieve reduced levels of toxicity, Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and ATG start codon. The specificity of the T7*(N4) to pK1F was achieved by engineering a DNA binding domain within the RNA polymerase.
 <!-- Add more about the biology of this part here