Difference between revisions of "Part:BBa K929100"

Latest revision as of 18:03, 1 October 2012

Anti-EGFR scFv425

General information

Anti-EGFR scFv425

BioBrick Nr.	BBa_929100
RFC standard	RFC 25
Requirement	pSB1C3
Source	plasmid pKMEF425bla
Submitted by	[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]

The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.

Characterization

For characterization and further applications see BBa_K929101.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 538
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K929100 short</partinfo>
+<p style="background-color: rgb(238, 221, 130); font-weight: bold;">General information</p>
 {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
-! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K929000 Anti-EGFR scFv425]
+! colspan="2" style="background:rgb(240, 20, 70);"|Anti-EGFR scFv425
 |-
-! colspan="2"|[[UP12_BBa_K929100_vector.png:|300px]]
+! colspan="2"|[[Image:UP12_BBa_K929100.png|300px]]
 |-
 |'''BioBrick Nr.'''
@@ Line 14: / Line 15: @@
 |-
 |'''Requirement'''
-|pSB1C3<br>
+|pSB1C3
 |-
 |'''Source'''
@@ Line 22: / Line 23: @@
 |[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]
 |}
+[[Image:UP12_BBa_K929100_vector.png|left|thumb|400px]]<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 <br>
-[[Image:UP12_BBa_K929100.png|left|thumb|400px]]<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
+The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection. <br>
-<br>
-The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.<br>
 <br>
-<!-- Add more about the biology of this part here
+<p style="background-color: rgb(238, 221, 130); font-weight: bold;">Characterization</p>
+For characterization and further applications see BBa_K929101.
 ===Usage and Biology===