Difference between revisions of "Part:BBa K782068"
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<partinfo>BBa_K782068 short</partinfo> | <partinfo>BBa_K782068 short</partinfo> | ||
| − | + | * TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011). | |
| + | * DNA binding sites for individual TAL effectors are indicated with square brackets [ ]. | ||
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| + | ==Introduction== | ||
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| + | Our construct contains four specific binding sites for [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782007 NicTAL12] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782005 TALD]. | ||
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| + | Single binding sequence for NicTAL12 is: TCTATCAATGATAGA | ||
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| + | Single binding sequence for TALD is: TCGTCCAATAGCTTCTC | ||
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| + | [[Image:4xNic+4xD.png]] | ||
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| + | '''Figure 1.''' Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD. | ||
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| + | * Binding sites for TAL effectors were ordered from IDT. | ||
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| + | |||
| + | ==Characterization== | ||
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| + | Specific TAL binding sites were further characterized with different reporter constructs. | ||
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| + | '''Results:''' | ||
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| + | * [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782025 BBa_K782025] | ||
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| + | ==References== | ||
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| + | Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53. | ||
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| + | Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698. | ||
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Latest revision as of 22:51, 26 September 2012
4x[NicTAL]+4x[TALD] operator
- TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
- DNA binding sites for individual TAL effectors are indicated with square brackets [ ].
Introduction
Our construct contains four specific binding sites for NicTAL12 and TALD.
Single binding sequence for NicTAL12 is: TCTATCAATGATAGA
Single binding sequence for TALD is: TCGTCCAATAGCTTCTC
Figure 1. Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD.
- Binding sites for TAL effectors were ordered from IDT.
Characterization
Specific TAL binding sites were further characterized with different reporter constructs.
Results:
References
Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 35
Illegal AgeI site found at 235 - 1000COMPATIBLE WITH RFC[1000]