Difference between revisions of "Part:BBa K929100"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K929100 short</partinfo>
 
<partinfo>BBa_K929100 short</partinfo>
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<p style="background-color: rgb(238, 221, 130); font-weight: bold;">General information</p>
  
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The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:rgb(240, 20, 70);"|Anti-EGFR scFv425
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|-
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! colspan="2"|[[Image:UP12_BBa_K929100.png|300px]]
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|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_929100 BBa_929100]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Requirement'''
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|pSB1C3
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|-
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|'''Source'''
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|plasmid pKMEF425bla
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|-
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|'''Submitted by'''
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|[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]
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|}
  
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<!-- Add more about the biology of this part here
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[[Image:UP12_BBa_K929100_vector.png|left|thumb|400px]]<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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<br>
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The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection. <br>
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<br>
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<p style="background-color: rgb(238, 221, 130); font-weight: bold;">Characterization</p>
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For characterization and further applications see BBa_K929101.
 
===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 18:03, 1 October 2012

Anti-EGFR scFv425

General information

Anti-EGFR scFv425
UP12 BBa K929100.png
BioBrick Nr. BBa_929100
RFC standard RFC 25
Requirement pSB1C3
Source plasmid pKMEF425bla
Submitted by [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]
UP12 BBa K929100 vector.png






















The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.

Characterization

For characterization and further applications see BBa_K929101.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 538
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]