Difference between revisions of "Part:BBa K771102"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K771102 short</partinfo>
 
<partinfo>BBa_K771102 short</partinfo>
  
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Inner membrane of ''E.coli''
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Inner membrane protein of ''E.coli''
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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To avoid toxicity caused by foreign membrane proteins, we chose phosphatidylglycerol:: prolipoprotein diacylglyceryl transferase (Lgt) as transmembrane domain in the project. Lgt is an inner membrane protein of E.coli with seven transmembrane segments and has been successfully over expressed in E. coli without causing harm to cells.
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To provide continence to the upperstream protein fusion, a flexible linker is added to the upperstream of lgt.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K771102 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K771102 SequenceAndFeatures</partinfo>

Latest revision as of 06:09, 28 September 2012

Lgt with Linker FL3

Inner membrane protein of E.coli

Usage and Biology

To avoid toxicity caused by foreign membrane proteins, we chose phosphatidylglycerol:: prolipoprotein diacylglyceryl transferase (Lgt) as transmembrane domain in the project. Lgt is an inner membrane protein of E.coli with seven transmembrane segments and has been successfully over expressed in E. coli without causing harm to cells. To provide continence to the upperstream protein fusion, a flexible linker is added to the upperstream of lgt.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 805
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 426