Difference between revisions of "Part:BBa K743018"

 
 
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<partinfo>BBa_K743018 short</partinfo>
 
<partinfo>BBa_K743018 short</partinfo>
  
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This is a circadian clock controlled reporter for Synechocystis PCC6803. Placed in the [https://parts.igem.org/Part:BBa_K743008 psb1C3_IntKR integrative plasmid] it has sfGFP with an C-terminal fast degradation tag under the control of the [https://parts.igem.org/Part:BBa_K743003 Pta promoter] which comes from a Synechocystis gene known to oscillate in a circadian manner.
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The short live of this tagged sfGFP allows for the caracterization of the activity of any promoter, in this case, the hypothesized oscillation of the Pta promoter.
  
  

Latest revision as of 21:50, 26 September 2012

sfGFP.degradation.tag under Pta promoter in psb1C3_IntKR plasmid

This is a circadian clock controlled reporter for Synechocystis PCC6803. Placed in the psb1C3_IntKR integrative plasmid it has sfGFP with an C-terminal fast degradation tag under the control of the Pta promoter which comes from a Synechocystis gene known to oscillate in a circadian manner.

The short live of this tagged sfGFP allows for the caracterization of the activity of any promoter, in this case, the hypothesized oscillation of the Pta promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2699
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2699
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2699
    Illegal XhoI site found at 3196
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2699
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2699
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511
    Illegal SapI.rc site found at 699