Difference between revisions of "Part:BBa K778010"

Latest revision as of 06:36, 6 October 2012

hycAp-GFPgenerator-plac-FhlAgenerator

You can "enhance" and "see" H2 production with this device.

hycAp-GFPgenerator-plac-FhlAgenerator

GFP is a reportor of H2 production (strictly speaking, reporter of hycAp activation Part:BBa K778000 (hycAp is the promotor of formate hydrogen lyase of genome)).

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from wild-type fhlA. This mutation increased H₂ production under the conditions in the previous research (Viviana et.al.2009). Actually, in our research (team UT-Tokyo 2012), this mutation increased H₂ production as the following figure. (The full construct in this experiment is Part:BBa K778008)

Detailed Information :Our wiki (UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] The results shown on the wiki are different from the latest result on this parts.igem page.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3266
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2545
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 828

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K778010 short</partinfo>
-hycAp-GFPgenerater-plac-FhlAgenerater
+You can "enhance" and "see" H2 production with this device.
+hycAp-GFPgenerator-plac-FhlAgenerator
+GFP is a reportor of H2 production (strictly speaking, reporter of  hycAp activation [[Part:BBa K778000]] (hycAp is the promotor of formate hydrogen lyase of genome)).
+FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] .
+E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
+fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from [[Part:BBa K778001|wild-type fhlA]].
+This mutation increased H<sub>2</sub>  production under the conditions in the previous research (Viviana et.al.2009).
+Actually, in our research (team UT-Tokyo 2012<!--[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]-->), this mutation increased H<sub>2</sub> production as the following figure.<!--this mutation did not increase H2 production.--> (The full construct in this experiment is [[Part:BBa K778008]])
+[[Image:UT Tokyo H2 1001+eg.png]]
+<!--[[Image:UT Tokyo H2 1001.png]]-->
+Detailed Information :Our wiki (UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
+The results shown on the wiki are different from the latest result on this parts.igem page.
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 32: @@
 <partinfo>BBa_K778010 parameters</partinfo>
 <!-- -->
+[[Image:Safety.png]]