Difference between revisions of "Part:BBa K785003"
 
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<partinfo>BBa_K785003 short</partinfo>
 
<partinfo>BBa_K785003 short</partinfo>
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This page described the Lux operon from Vibrio fischeri. Lux gene is directly PCRed from the BBa_K325909, the pbad operon is deleted and now this part is available for any expression unit. You can provide light out put by putting this coding device under your promoter.
 
This page described the Lux operon from Vibrio fischeri. Lux gene is directly PCRed from the BBa_K325909, the pbad operon is deleted and now this part is available for any expression unit. You can provide light out put by putting this coding device under your promoter.
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We have successfully put this coding sequence into the PET28a expression system (cell strain is dH5&#945;), and light out put could induced by the IPTG.
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We have successfully put this coding sequence into the PET28a expression system (cell strain is BL21 DE3), and light out put could be induced by the IPTG.
 
To see more information about Lux light generator, please visit [https://parts.igem.org/Part:BBa_K325909 BBa_K325909], and a more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2010 Cambridge iGEM].
 
To see more information about Lux light generator, please visit [https://parts.igem.org/Part:BBa_K325909 BBa_K325909], and a more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2010 Cambridge iGEM].
  
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K785003 SequenceAndFeatures</partinfo>
  
 
[[Image:LUX expression 3.jpg|thumb|569px|center|The Lux light generator part expressed in the PET28a system(under the T7 lacO control). The Lux is induced by IPTG.]]
 
[[Image:LUX expression 3.jpg|thumb|569px|center|The Lux light generator part expressed in the PET28a system(under the T7 lacO control). The Lux is induced by IPTG.]]

Latest revision as of 13:11, 26 September 2012

Lux(CDABEG) coding gene PoPS->Light

This page described the Lux operon from Vibrio fischeri. Lux gene is directly PCRed from the BBa_K325909, the pbad operon is deleted and now this part is available for any expression unit. You can provide light out put by putting this coding device under your promoter. We have successfully put this coding sequence into the PET28a expression system (cell strain is BL21 DE3), and light out put could be induced by the IPTG. To see more information about Lux light generator, please visit BBa_K325909, and a more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2010 Cambridge iGEM].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1959
    Illegal XhoI site found at 2789
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4348
    Illegal BsaI.rc site found at 1357
    Illegal SapI.rc site found at 4673
The Lux light generator part expressed in the PET28a system(under the T7 lacO control). The Lux is induced by IPTG.