Difference between revisions of "Part:BBa K743015:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K743015 short</partinfo>
 
<partinfo>BBa_K743015 short</partinfo>
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===Source===
 
===Source===
  
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Pta promoter was amplified from genomic Synechocystis DNA, you can find a biobrick version of this promoter at blahblahblah.
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Pta promoter was amplified from genomic Synechocystis DNA, you can find references and more information at the [https://parts.igem.org/Part:BBa_K743003 biobrick version of this promoter].
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LuxAB genes were obtained from the Luxbrick
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LuxAB genes were obtained from the [https://parts.igem.org/Part:BBa_K325909 Luxbrick]
  
 
===References===
 
===References===

Latest revision as of 21:14, 22 September 2012

after dusk -> LuxAB luciferase (Vibrio fischerii)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 693
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 521
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2080


Design Notes

This part was made by Gibson assembly, therefore it has no scars


Source

Pta promoter was amplified from genomic Synechocystis DNA, you can find references and more information at the biobrick version of this promoter. LuxAB genes were obtained from the Luxbrick

References