Difference between revisions of "Part:BBa K861169"
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<partinfo>BBa_K861169 short</partinfo> | <partinfo>BBa_K861169 short</partinfo> | ||
| − | This is an indirect regulatory devices activated at high glucose concentration through an intermediate protein cI of lambda phage. In this pathway, protein cI will be expressed when CRP is activated at low glucose concentration. cI will therefore repress the expression of gene downstream the promoter BBa_R0051 heavily. On the contrary, when glucose concentration is high, the promoter will be derepressed. | + | This is an indirect regulatory devices activated at high glucose concentration through an intermediate protein cI of lambda phage. In this pathway, protein cI will be expressed when CRP is activated at low glucose concentration. cI will therefore repress the expression of gene downstream the promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_R0051 BBa_R0051] heavily. On the contrary, when glucose concentration is high, the promoter will be derepressed. |
There was no promoter existing in the nature which is directly activated by glucose. In this device, RFP was designed as a reporter. RFP can be placed by an arbitrary protein that needs to be activated by glucose. | There was no promoter existing in the nature which is directly activated by glucose. In this device, RFP was designed as a reporter. RFP can be placed by an arbitrary protein that needs to be activated by glucose. | ||
Latest revision as of 19:09, 26 September 2012
Indirect regulatory device, activated at high glucose concentration
This is an indirect regulatory devices activated at high glucose concentration through an intermediate protein cI of lambda phage. In this pathway, protein cI will be expressed when CRP is activated at low glucose concentration. cI will therefore repress the expression of gene downstream the promoter BBa_R0051 heavily. On the contrary, when glucose concentration is high, the promoter will be derepressed. There was no promoter existing in the nature which is directly activated by glucose. In this device, RFP was designed as a reporter. RFP can be placed by an arbitrary protein that needs to be activated by glucose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1673
Illegal AgeI site found at 1785 - 1000COMPATIBLE WITH RFC[1000]