Difference between revisions of "Part:BBa K733010:Design"
(→Design Notes) |
(→Source) |
||
| (One intermediate revision by one other user not shown) | |||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | + | We digest ''Ptms'' and RBS+''ydcD'' and ligate them. | |
| + | |||
| + | Backbone: ''Ptms'' in pSB1C3. Enzyme used: SpeI and PstI-HF. | ||
| + | |||
| + | Insert: RBS+''ydcD''. Enzyme used: XbaI and PstI-HF. Purified by gel purification. | ||
===References=== | ===References=== | ||
Latest revision as of 16:34, 26 September 2012
Ptms+RBS+ydcD.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
We digest Ptms and RBS+ydcD and ligate them.
Backbone: Ptms in pSB1C3. Enzyme used: SpeI and PstI-HF.
Insert: RBS+ydcD. Enzyme used: XbaI and PstI-HF. Purified by gel purification.