Difference between revisions of "Part:BBa K733003:Design"

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===Design Notes===
 
===Design Notes===
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To easily building an antitoxin expression device, forward primer containing consensus RBS sequence in ''B. subtilis'' is designed, aiming at adding RBS directly to the upstream of ydcD gene through PCR.
+
To easily building an antitoxin expression device, forward primer containing consensus RBS sequence in ''B. subtilis'' is designed, aiming at adding RBS directly to the upstream of ''ydcD'' gene through PCR.
  
 
===Source===
 
===Source===
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We obtain this part from the genomic DNA of ''Bacillus subtilis 168'' via PCR amplification.
 
We obtain this part from the genomic DNA of ''Bacillus subtilis 168'' via PCR amplification.
  
−
Forward primer: 5’-aaaggaggtgtatgttttttgtctg-3’ (Prefix not shown)
+
Forward primer: 5’-AAAGGAGGTGTATGTTTTTTGTCTG-3’ (Prefix not shown)
  
 
Reverse primer: 5’-TGATTATCCTCCGCTGACTA-3’ (Suffix not shown)
 
Reverse primer: 5’-TGATTATCCTCCGCTGACTA-3’ (Suffix not shown)
  
 
===References===
 
===References===

Latest revision as of 19:47, 26 September 2012

RBS+ydcD: antitoxin gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To easily building an antitoxin expression device, forward primer containing consensus RBS sequence in B. subtilis is designed, aiming at adding RBS directly to the upstream of ydcD gene through PCR.

Source

We obtain this part from the genomic DNA of Bacillus subtilis 168 via PCR amplification.

Forward primer: 5’-AAAGGAGGTGTATGTTTTTTGTCTG-3’ (Prefix not shown)

Reverse primer: 5’-TGATTATCCTCCGCTGACTA-3’ (Suffix not shown)

References