Difference between revisions of "Part:BBa K743011"

Latest revision as of 20:00, 24 September 2012

OmpA + HIV protease cleavage site secretion system

This device is designed for the further incorporation of any protein coding sequence downstream the HIV cleavage site. Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker (ompA brick) followed by a cleavage site recognizable by HIV protease.

If any polypeptide sequence is fused to the 3' end of the HIV cleavage site DNA sequence, it will protrude from the outer membrane where it will be cleaved from the ompA domain by HIV protease.

This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP and a spider silk monomer.

You can check the results HERE LINK!

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K743011 short</partinfo>
+This device is designed for the further incorporation of any protein coding sequence downstream the HIV cleavage site.
+Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A  fused to a extracellular linker ([https://parts.igem.org/Part:BBa_K103006 ompA brick]) followed by a cleavage site recognizable by HIV protease.
+If any polypeptide sequence is fused to the 3' end of the HIV cleavage site DNA sequence, it will protrude from the outer membrane where it will be cleaved from the ompA domain by HIV protease.
+This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP and a spider silk monomer.
+You can check the results HERE LINK!
 <partinfo>BBa_K743011 SequenceAndFeatures</partinfo>