Difference between revisions of "Part:BBa K899003"

Latest revision as of 02:15, 27 September 2012

Reporter system for FFT (BBa_K899001)

Reporter system for characterization of fructan fructan fructosyltransferase (FFT) gene transcription, with green flourescent protein (GFP) as reporter and lacl as promoter. This construct was designed with the promoter BBa_R0010 in order to control transcription. We suspect transcription of 1-FFT to greatly inhibit bacterial growth with the production of plant inulin. LacI protein and CAP can succesfully inhibit transcription of both genes to allow the bacteria to grow. With the addition of IPTG to the medium, LacI will be inhibited and transcription can resume.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 721
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 575
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2725

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K899003 short</partinfo>
-Reporter system for characterization of fructan fructan fructosyltransferase (FFT) gene transcription, with green flourescent protein (GFP) as reporter and lacl as promoter
+Reporter system for characterization of fructan fructan fructosyltransferase (FFT) gene transcription, with green flourescent protein (GFP) as reporter and lacl as promoter.
+This construct was designed with the promoter BBa_R0010 in order to control transcription. We suspect transcription of 1-FFT to greatly inhibit bacterial growth with the production of plant inulin. LacI protein and CAP can succesfully inhibit transcription of both genes to allow the bacteria to grow. With the addition of IPTG to the medium, LacI will be inhibited and transcription can resume.
 <!-- Add more about the biology of this part here