Difference between revisions of "Part:BBa K896006"
 
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<partinfo>BBa_K896006 short</partinfo>
 
<partinfo>BBa_K896006 short</partinfo>
  
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Cloning of SQR gene:
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Cloning of NirN+NirS gene:
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SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.<p></p>  
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Nitrite reductase is composed of several different subunits. Together, it can reduce nitrite to nitric oxide. We clone all these subunits together on the same plasmid in order to grant cyanobacteria a functional protein.
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[[Image:NirN+S cloning.jpg]]
 
[[Image:NirN+S cloning.jpg]]
  

Latest revision as of 17:34, 25 September 2012

NirN-NirS (Nitrite->Nitric oxide reductase)

Cloning of NirN+NirS gene:

Nitrite reductase is composed of several different subunits. Together, it can reduce nitrite to nitric oxide. We clone all these subunits together on the same plasmid in order to grant cyanobacteria a functional protein.

NirN+S cloning.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1703
    Illegal BamHI site found at 1483
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 168
    Illegal NgoMIV site found at 220
    Illegal NgoMIV site found at 604
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 2305
    Illegal AgeI site found at 3169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2347
    Illegal BsaI.rc site found at 2479