Difference between revisions of "Part:BBa K817050:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K817003 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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In the paper, the clone the promoter upstream from transcription starting site till -85 region; however, we only clone up till -45 regions, in which -45~-35 region is proven to be critical to the promoter function.  
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In the paper, the clone the promoter upstream from transcription starting site till -85 region; however, we only clone up till -45 regions, in which -45~-35 region is proven to be critical to the promoter function.
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For our functional assay result, please refer to our [http://2012.igem.org/Team:NTU-Taida/Result/Thermal-Promoter wiki page].
  
 
===Source===
 
===Source===
  
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"Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase", Cell press, 1984
 
ATGCTGCCACCCTTGAAAAACTGTCGATGTGGGACGATATAGCAGAT(+1)
 
ATGCTGCCACCCTTGAAAAACTGTCGATGTGGGACGATATAGCAGAT(+1)
  
 
===References===
 
===References===

Latest revision as of 01:56, 27 September 2012

Novel thermal promoter, Phs


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the paper, the clone the promoter upstream from transcription starting site till -85 region; however, we only clone up till -45 regions, in which -45~-35 region is proven to be critical to the promoter function.

For our functional assay result, please refer to our [http://2012.igem.org/Team:NTU-Taida/Result/Thermal-Promoter wiki page].

Source

"Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase", Cell press, 1984 ATGCTGCCACCCTTGAAAAACTGTCGATGTGGGACGATATAGCAGAT(+1)

References