Difference between revisions of "Part:BBa K785000:Design"

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This coding sequence is form the  Avena sativa phototrophin1(AsLOV2) and tetracycline  resistance. The whole gene is synthesized by Invitrogen company.
 
This coding sequence is form the  Avena sativa phototrophin1(AsLOV2) and tetracycline  resistance. The whole gene is synthesized by Invitrogen company.
  
−
[[Image:Lov_fmn_binding.PNG|left|Structure of this protein. Shows the binding between protein and DNA.]]
+
[[Image:Lov_fmn_binding.PNG|frame|left|Lov domain, shows the binding with ligand FMN]]
 +
 
 +
[[Image:TetR.png|frame|left|tetR domain, shows the binding between protein and DNA]]
  
 
===References===
 
===References===
 +
Review about tetR domain:[http://mmbr.asm.org/content/69/2/326.short The TetR family of transcriptional repressors]
 +
 +
Review about Lov domain: [http://www.nature.com/nrmicro/journal/v9/n10/abs/nrmicro2622.html Function, structure and mechanism of bacterial photosensory LOV proteins]

Latest revision as of 06:28, 22 September 2012

Lov-tetR (light sensor with Ptet repression) light->PtetO repressed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
    Illegal AgeI site found at 478
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed for our biowave project. See more about Biowave visit our wiki:[http://2012.igem.org/Team:Fudan_Lux Fudan_Lux 2012]

Source

This coding sequence is form the Avena sativa phototrophin1(AsLOV2) and tetracycline resistance. The whole gene is synthesized by Invitrogen company.

Lov domain, shows the binding with ligand FMN
tetR domain, shows the binding between protein and DNA

References

Review about tetR domain:[http://mmbr.asm.org/content/69/2/326.short The TetR family of transcriptional repressors]

Review about Lov domain: [http://www.nature.com/nrmicro/journal/v9/n10/abs/nrmicro2622.html Function, structure and mechanism of bacterial photosensory LOV proteins]