Difference between revisions of "Part:BBa K785000"
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This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light.
 
This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light.
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This protein, which is actually a well designed fusion protein, include two domain. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline  resistance, which could combine the tetracycline and enable the expression of the tetA.  
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This protein, which is actually a well designed fusion protein, includes two domains. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline  resistance, which could combine the tetracycline and enable the expression of the tetA.  
 
We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.  
 
We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.  
  
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<partinfo>BBa_K785000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K785000 SequenceAndFeatures</partinfo>
  
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[[Image:Lov-TetR.png|frame|left|Structure of this protein. Shows the binding between protein and DNA.]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 21:13, 15 September 2014

Lov-tetR (light sensor with Ptet repression) light->PtetO repressed

This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light. This protein, which is actually a well designed fusion protein, includes two domains. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline resistance, which could combine the tetracycline and enable the expression of the tetA. We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 106
    Illegal PstI site found at 160
    Illegal PstI site found at 289
    Illegal PstI site found at 626
    Illegal PstI site found at 940
    Illegal PstI site found at 1033
    Illegal AgeI site found at 478
  • 1000
    COMPATIBLE WITH RFC[1000]
Structure of this protein. Shows the binding between protein and DNA.