Difference between revisions of "Part:BBa K896006:Design"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K896006 short</partinfo>
 
<partinfo>BBa_K896006 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
YES
 
−
 
  
 +
Cloning of NirN-NirS gene:
 +
SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.<p></p>
 +
[[Image:NirN+S cloning.jpg]]
  
 
===Source===
 
===Source===

Latest revision as of 12:40, 22 September 2012

NirN-NirS (Nitrite->Nitric oxide reductase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1703
    Illegal BamHI site found at 1483
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 168
    Illegal NgoMIV site found at 220
    Illegal NgoMIV site found at 604
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 2305
    Illegal AgeI site found at 3169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2347
    Illegal BsaI.rc site found at 2479


Design Notes

Cloning of NirN-NirS gene:

SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.

NirN+S cloning.jpg

Source

YES

References