Difference between revisions of "Part:BBa K750114"

 
 
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<partinfo>BBa_K750114 short</partinfo>
 
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This part contains ''PtetcIT''(BBa_K750102) and ''PcIGLT''(BBa_K750103). Atc can activate the promoter ''pBAD'' and start up expression of cI protein. Protein cI will activated promoter ''PcI'' to repress the expression of GFP(lva). We use this part to make a bright cell turn to dark, to meet demand of number switching. 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:30, 26 September 2012

PtetcIT-PcIGLT: atc->cI->GFP(lva)

This part contains PtetcIT(BBa_K750102) and PcIGLT(BBa_K750103). Atc can activate the promoter pBAD and start up expression of cI protein. Protein cI will activated promoter PcI to repress the expression of GFP(lva). We use this part to make a bright cell turn to dark, to meet demand of number switching.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2610