Difference between revisions of "Part:BBa K861074:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K861074=== | ===Applications of BBa_K861074=== | ||
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| + | <p align="justify">In our experiment, cells that expressed protein which was nothing to do with cellulose synthesis was used as a control.We transformed the seven genes involved in cellulose synthesis into <em>E.coli</em> and meaaured cellulose production. In the step of measuring total cellulose production, exceed cellulase was appended and incubated at 50 ℃ for 1 hour, and results show that the overexpression of adrA can help increase the ability of cellulose synthesis.</p> | ||
| + | <p align="center"><img src="https://static.igem.org/mediawiki/2012/a/a7/CELLULOSE_PRODUCTION.tif" alt="" name="CelluloseFig1" width="520" height="412" align="middle" id="CelluloseFig1" /></p> | ||
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| + | <p align="justify">And also we co-overexpress adrA with bcsA. Cellulose output in both supernatant and deposit were show in the following figure. The cellulose production in <em>adrA</em>+<em>bcsA</em> is more than 0.6 mg/mL, which is almost two times higher than that of control.</p> | ||
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| + | <p align="center"><img src="https://static.igem.org/mediawiki/igem.org/f/ff/Cellulose_Fig_1.jpg" alt="" name="CelluloseFig1" width="520" height="1247" align="middle" id="CelluloseFig1" /></p> | ||
| + | <p>All these results indicated that AdrA can increase the ability of cellulose synthesis.Actually, BcsB can be activated by c-di-GMP. But till the deadline, we have not successfully assembled Pcar and bcsB. In the following work, we are going to assemble a plasmid including all of the seven genes. Maybe in this way, cellulose production will increase greatly.</p> | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 16:52, 26 October 2012
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K861074
In our experiment, cells that expressed protein which was nothing to do with cellulose synthesis was used as a control.We transformed the seven genes involved in cellulose synthesis into E.coli and meaaured cellulose production. In the step of measuring total cellulose production, exceed cellulase was appended and incubated at 50 ℃ for 1 hour, and results show that the overexpression of adrA can help increase the ability of cellulose synthesis.
And also we co-overexpress adrA with bcsA. Cellulose output in both supernatant and deposit were show in the following figure. The cellulose production in adrA+bcsA is more than 0.6 mg/mL, which is almost two times higher than that of control.
All these results indicated that AdrA can increase the ability of cellulose synthesis.Actually, BcsB can be activated by c-di-GMP. But till the deadline, we have not successfully assembled Pcar and bcsB. In the following work, we are going to assemble a plasmid including all of the seven genes. Maybe in this way, cellulose production will increase greatly.
User Reviews
UNIQc92f3f1be0be6446-partinfo-00000001-QINU UNIQc92f3f1be0be6446-partinfo-00000002-QINU