Difference between revisions of "Part:BBa K784019"
 
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This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br>
 
This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br>
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Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome. <br>
+
Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome. <br>
−
Fragment 5 contains the recombination area that allows the phage lysogenic life cycle (penetration to the host genome). This part includes the att-λ sequence which allows site specific recombination into an E. coli cell (with the matching att-B sequence) between gal and bio operons. <br>
+
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here]. <br>
 +
Fragment 5 contains the recombination area that allows the phage lysogenic life cycle (penetration to the host genome). This part includes the att-λ sequence which allows site specific recombination into an ''E. coli'' genome (with the matching att-B sequence) between gal and bio operons. <br>
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 19:32, 26 September 2012

Phage Lambda fragment 5 - recombination

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome.
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here].
Fragment 5 contains the recombination area that allows the phage lysogenic life cycle (penetration to the host genome). This part includes the att-λ sequence which allows site specific recombination into an E. coli genome (with the matching att-B sequence) between gal and bio operons.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4318
    Illegal PstI site found at 4576
    Illegal PstI site found at 4823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4318
    Illegal PstI site found at 4576
    Illegal PstI site found at 4823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4318
    Illegal BamHI site found at 543
    Illegal XhoI site found at 6069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4318
    Illegal PstI site found at 4576
    Illegal PstI site found at 4823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4318
    Illegal PstI site found at 4576
    Illegal PstI site found at 4823
    Illegal AgeI site found at 4396
  • 1000
    COMPATIBLE WITH RFC[1000]