Difference between revisions of "Part:BBa K861003"
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<partinfo>BBa_K861003 short</partinfo> | <partinfo>BBa_K861003 short</partinfo> | ||
− | Long-chain fatty acids are transported across the cell membrane via a | + | Long-chain fatty acids are transported across the cell membrane via a transportation/acyl-activation mechanism involving an outer membrane protein, FadL(BBa_K861500). The FadL gene encodes a 43,000-dalton membrane protein (FLP) which has been implicated as being essential for LCFA transportation. When expressed, FadL can transport the long-chain fatty acids into the bacterium. We assembled FadL with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high. |
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Latest revision as of 13:20, 22 September 2012
PfadR regulated FadL
Long-chain fatty acids are transported across the cell membrane via a transportation/acyl-activation mechanism involving an outer membrane protein, FadL(BBa_K861500). The FadL gene encodes a 43,000-dalton membrane protein (FLP) which has been implicated as being essential for LCFA transportation. When expressed, FadL can transport the long-chain fatty acids into the bacterium. We assembled FadL with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1207
- 1000COMPATIBLE WITH RFC[1000]