Difference between revisions of "Part:BBa K801095:Design"

 
(→‎Design Notes)
 
(6 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K801095 short</partinfo>
 
<partinfo>BBa_K801095 short</partinfo>
Line 5: Line 4:
 
<partinfo>BBa_K801095 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K801095 SequenceAndFeatures</partinfo>
  
 +
 +
Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA
 +
 +
http://www.ncbi.nlm.nih.gov/nucleotide/11096318/
 +
 +
PMID: 15932991
 +
 +
<br>'''Keywords:'''
 +
Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, aromatic prenyltransferase
 +
 +
<br>'''Abbreviations:'''
 +
CHS = naringenin-chalcone synthase
  
 
===Design Notes===
 
===Design Notes===
−
-
 
  
 +
'''Related BioBrick:'''
  
 +
Other versions: [https://parts.igem.org/Part:BBa_K801094 BBa_K801094: CHS + yeast consensus sequence]
 +
 +
Related BioBricks:
 +
* [https://parts.igem.org/Part:BBa_K801090 BBa_K801090: phenylalanine ammonia lyase (PAL) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801091 BBa_K801091: phenylalanine ammonia lyase (PAL) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801092 BBa_K801092: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801093 BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801094 BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801096 BBa_K801096: Aromatic prenyltransferase (APT) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801097 BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801098 BBa_K801098: O-methyltransferase 1 (OMT1) coding region]
 +
 +
 +
'''Cloning details:'''<br>
 +
Designed in RFC10/RFC25
 +
<!--*Mutation C889G to delete XbaI restriction site-->
 +
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 +
 +
'''Quality control measures:'''<br>
 +
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 +
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 +
Part was totally sequenced
 +
 +
'''Backbone:'''<br>
 +
*Backbone name: pSB1C3
 +
*Resistance: Cp
 +
*Copynumber: high
 +
 +
'''Protein coding:'''<br>
 +
*Protein: naringenin-chalcone synthase
 +
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 +
<!--*The protein encoded is posttranslationally modified by ???.-->
 +
*Tag: c-terminally fused Strep
 +
 +
'''Enzymatic activity:'''<br>
 +
EC-number: 2.3.1.74
 +
 +
'''Cytotoxicity:'''<br>
 +
<!--none/not known/cytotoxic for ''organism name''-->
 +
 +
'''Safety notes:'''<br>
 +
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 +
<!--Known and anticipated security issues: none/other.-->
 +
 +
'''Intellectual property:'''
 +
<!--Information on patent situation.-->
 +
<!--Intellectual property claims made by the authors.-->
 +
 +
'''Corresponding part author/authors:'''
 +
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
−
Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA
+
'''Source:'''<br>
 +
<!--*Commercial system: plasmid name, system name, company name-->
 +
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA
 +
<!--*Preexisting BioBrick ?Bba_number?-->
 +
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
 +
<!--*Synthesized by ?company_name?.-->
  
−
http://www.ncbi.nlm.nih.gov/nucleotide/11096318/
+
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
  
−
PMID: 15932991
+
'''Organism:'''<br>
 +
Genesequence derived from ''Hypericum androsaemum''
 +
<!--*Codonoptimized for ''?organism_name?''-->
 +
<!--*Designed for the following Chassis: ''?organism-name?''-->
 +
<!--*Statement about functionality in other chassis.-->
  
 
===References===
 
===References===
 +
<!-- Here you find templates to insert references to literature and different databases-->
 +
 +
'''Literature references:'''<br>
 +
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 '''Pubmed''' : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]
 +
 +
 +
'''Database references:'''<br>
 +
[http://www.ncbi.nlm.nih.gov/nuccore/AF315345.1 '''GenBank''': Hypericum androsaemum chalcone synthase mRNA, complete cds]
 +
 +
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
 +
[http://www.uniprot.org/uniprot/Q9FUB7  '''Uniprot''': Chalcone synthase]
 +
<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 +
<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 +
<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 12:18, 3 October 2012

Naringenin - chalcone synthase (CHS) coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 717
    Illegal BsaI.rc site found at 1138
    Illegal SapI.rc site found at 840


Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/11096318/

PMID: 15932991


Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, aromatic prenyltransferase


Abbreviations: CHS = naringenin-chalcone synthase

Design Notes

Related BioBrick:

Other versions: BBa_K801094: CHS + yeast consensus sequence

Related BioBricks:


Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: naringenin-chalcone synthase
  • Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: 2.3.1.74

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA


Organism:
Genesequence derived from Hypericum androsaemum

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]


Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AF315345.1 GenBank: Hypericum androsaemum chalcone synthase mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q9FUB7 Uniprot: Chalcone synthase]