Difference between revisions of "Part:BBa K801011:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K801011 short</partinfo>
 
<partinfo>BBa_K801011 short</partinfo>
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===Design Notes===
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Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
 
Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
  
  
  
−
===Source===
+
 
  
 
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
 
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
 +
 +
<br>'''Keywords:'''
 +
<!--These keywords are necessary to find your part using a fulltext sarch.-->
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<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
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<br>'''Abbreviations:'''
 +
<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
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<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
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===Design Notes===
 +
 +
'''Related BioBrick:'''
 +
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
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<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 +
 +
'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25/RFC21
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* Mutation in NotI in Suffix
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<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
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<!--*Mutation C889G to delete XbaI restriction site-->
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<!--*Truncation upstream/downstream compared to template, ?explanation?-->
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'''Quality control measures:'''<br> Test digestion using XbaI+PstI-HF, EcorI+ SpeI-HF
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*Sequencing using primer VR and VF2
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<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
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<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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<!--*Part was partly sequenced/Part was totally sequenced-->
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'''Backbone:'''<br> Backbone name: pSB1C3
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<!--*Backbone name: pSB1C3'/?backbone_name?-->
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<!--*Resistance: Amp/Cp/Kan/Tet-->
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<!--*Copynumber: low/medium/high-->
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 +
'''Protein coding:'''<br>
 +
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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<!--*The protein encoded is posttranslationally modified by ???.-->
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
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 +
'''Enzymatic activity:'''
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<!--none/EC-number ?.?.?.?-->
 +
 +
'''Cytotoxicity:'''<br> none
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<!--none/not known/cytotoxic for ''organism name''-->
 +
 +
'''Safety notes:'''<br> none
 +
<!--Known and anticipated safety issues: none/health_risk/environmental_risk/other_risk-->
 +
<!--Known and anticipated security issues: none/other.-->
 +
 +
'''Intellectual property:'''
 +
<!--Information on patent situation.-->
 +
<!--Intellectual property claims made by the authors.-->
 +
 +
'''Corresponding part author/authors:'''
 +
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
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 +
===Source===
 +
 +
'''Source:'''<br> PCR product from genomic DNA of ''Saccharomyces cerevisiae''
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<!--*Commercial system: plasmid name, system name, company name-->
 +
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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<!--*Preexisting BioBrick ?Bba_number?-->
 +
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
 +
<!--*Synthesized by ?company_name?.-->
 +
 +
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
 +
'''Organism:'''<br> ''Saccharomyces cerevisiae''
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<!--*Genesequence derived from ''?organism_name?''-->
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<!--*Codonoptimized for ''?organism_name?''-->
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<!--*Designed for the following Chassis: ''?organism-name?''-->
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<!--*Statement about functionality in other chassis.-->
  
 
===References===
 
===References===
 +
<!-- Here you find templates to insert references to literature and different databases-->
 +
 +
'''Literature references:'''<br>
 +
*Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.
 +
*Guo, Z.; Sherman, F. (1996): 3'-end-forming signals of yeast mRNA. In: Trends Biochem. Sci. 21 (12), S. 477–481.
 +
 +
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 +
 +
'''Database references:'''<br>
 +
 +
<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
 +
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
 +
<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
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<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
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<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
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<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 22:45, 26 September 2012

TEF1 yeast terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.



Extracted from genomic DNA of S. cerevisiae using PCR.


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:
Test digestion using XbaI+PstI-HF, EcorI+ SpeI-HF

  • Sequencing using primer VR and VF2

Backbone:
Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:
none

Safety notes:
none

Intellectual property:

Corresponding part author/authors:

Source

Source:
PCR product from genomic DNA of Saccharomyces cerevisiae


Organism:
Saccharomyces cerevisiae

References

Literature references:

  • Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.
  • Guo, Z.; Sherman, F. (1996): 3'-end-forming signals of yeast mRNA. In: Trends Biochem. Sci. 21 (12), S. 477–481.


Database references: