Difference between revisions of "Part:BBa K801010:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K801010 short</partinfo>
 
<partinfo>BBa_K801010 short</partinfo>
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<partinfo>BBa_K801010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K801010 SequenceAndFeatures</partinfo>
  
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Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
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<br>'''Keywords:'''
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<!--These keywords are necessary to find your part using a fulltext sarch.-->
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<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
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<br>'''Abbreviations:'''
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<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
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<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
  
 
===Design Notes===
 
===Design Notes===
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'''Related BioBrick:'''[https://parts.igem.org/wiki/index.php?title=Part:BBaBBa_K165037: BBa_K165037]
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<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
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<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
  
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'''Cloning details:'''<br> Designed in RFC10/RFC23
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<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
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<!--*Mutation C889G to delete XbaI restriction site-->
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<!--*Truncation upstream/downstream compared to template, ?explanation?-->
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'''Quality control measures:'''<br> Test digestion using XbaI+ PstI-HF, SpeI-HF+ PstI-HF
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<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
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<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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<!--*Part was partly sequenced/Part was totally sequenced-->
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'''Backbone:'''<br> Backbone name: pSB1C3, Resistance: Cp
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<!--*Backbone name: pSB1C3'/?backbone_name?-->
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<!--*Resistance: Amp/Cp/Kan/Tet-->
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<!--*Copynumber: low/medium/high-->
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'''Protein coding:'''<br>
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<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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<!--*The protein encoded is posttranslationally modified by ???.-->
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
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'''Enzymatic activity:'''
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<!--none/EC-number ?.?.?.?-->
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'''Cytotoxicity:'''<br> none
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<!--none/not known/cytotoxic for ''organism name''-->
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'''Safety notes:'''<br> none
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<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
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<!--Known and anticipated security issues: none/other.-->
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'''Intellectual property:'''
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<!--Information on patent situation.-->
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<!--Intellectual property claims made by the authors.-->
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'''Corresponding part author/authors:'''
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<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
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Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
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'''Source:'''<br> PCR from genomic DNA of ''Saccharomyces cerevisiae''
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<!--*Commercial system: plasmid name, system name, company name-->
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<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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<!--*Preexisting BioBrick ?Bba_number?-->
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<!--*cDNA Clone: ?clone_name?, ?company_name?-->
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<!--*Synthesized by ?company_name?.-->
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* Forward Primer: VF2
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* Reverse Primer: VR
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<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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'''Organism:'''<br> Developed for ''Saccharomyces cerevisiae''
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<!--*Genesequence derived from ''?organism_name?''-->
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<!--*Codonoptimized for ''?organism_name?''-->
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<!--*Designed for the following Chassis: ''?organism-name?''-->
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<!--*Statement about functionality in other chassis.-->
  
 
===References===
 
===References===
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<!-- Here you find templates to insert references to literature and different databases-->
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'''Literature references:'''<br>
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*Schirmaier, F.; Philippsen, P. (1984): Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae. In: EMBO J. 3 (13), S. 3311–3315.
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*Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.
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<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
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'''Database references:'''<br>
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*http://ypa.ee.ncku.edu.tw/index.html
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<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
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<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
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<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
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<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
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<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
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<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 22:02, 26 September 2012

TEF2 constitutive yeast promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
  • 1000
    COMPATIBLE WITH RFC[1000]




Extracted from genomic DNA of S. cerevisiae using PCR.


Keywords:


Abbreviations:

Design Notes

Related BioBrick:BBa_K165037

Quality control measures:
Test digestion using XbaI+ PstI-HF, SpeI-HF+ PstI-HF

Backbone:
Backbone name: pSB1C3, Resistance: Cp

Protein coding:

Enzymatic activity:

Cytotoxicity:
none

Safety notes:
none

Intellectual property:

Corresponding part author/authors:

Source

Source:
PCR from genomic DNA of Saccharomyces cerevisiae

  • Forward Primer: VF2
  • Reverse Primer: VR

Organism:
Developed for Saccharomyces cerevisiae

References

Literature references:

  • Schirmaier, F.; Philippsen, P. (1984): Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae. In: EMBO J. 3 (13), S. 3311–3315.
  • Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.


Database references:

  • http://ypa.ee.ncku.edu.tw/index.html