Difference between revisions of "Part:BBa K801003"

Latest revision as of 21:03, 26 September 2012

pTUM103 yeast shuttle vector with pADH1 promoter

This part corresponds to pTUM100 (BBa_K801000). The constitutive pADH1 promoter has been cloned into the MCS (RFC25 prefix and suffix). Part is compatible with RFC 10 and RFC 25

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 36
Illegal suffix found at 776
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal NheI site found at 4041
Illegal SpeI site found at 777
Illegal PstI site found at 791
Illegal NotI site found at 42
Illegal NotI site found at 784
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 36
Illegal suffix found at 777
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 36
Plasmid lacks a suffix.
Illegal XbaI site found at 51
Illegal SpeI site found at 777
Illegal PstI site found at 791
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 149
Illegal BsaI.rc site found at 2198
Illegal BsaI.rc site found at 3144
Illegal SapI site found at 1115
Illegal SapI site found at 3296

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K801003 short</partinfo>
-This part corresponds to pTUM100 (BBa_K801000). The constitutive pADH1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
+This part corresponds to pTUM100 (BBa_K801000). The constitutive pADH1 promoter has been cloned into the MCS (RFC25 prefix and suffix).
+'''Part is compatible with RFC 10 and RFC 25'''
 <!-- Add more about the biology of this part here