Difference between revisions of "Part:BBa K801002"

Latest revision as of 21:23, 26 September 2012

pTUM102 yeast shuttle vector with pTEF2 promoter

This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF2 promoter has been into the MCS (RFC25 prefix and suffix). Part is compatible with RFC 10

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 36
Illegal suffix found at 705
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal NheI site found at 3970
Illegal SpeI site found at 706
Illegal PstI site found at 720
Illegal NotI site found at 42
Illegal NotI site found at 713
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 36
Illegal suffix found at 706
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 36
Plasmid lacks a suffix.
Illegal XbaI site found at 51
Illegal SpeI site found at 706
Illegal PstI site found at 720
Illegal NgoMIV site found at 194
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2127
Illegal BsaI.rc site found at 3073
Illegal SapI site found at 1044
Illegal SapI site found at 3225

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K801002 short</partinfo>
-This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF2 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
+This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF2 promoter has been into the MCS (RFC25 prefix and suffix).
+'''Part is compatible with RFC 10'''
 <!-- Add more about the biology of this part here