Difference between revisions of "Part:BBa K861177"
 
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<partinfo>BBa_K861177 short</partinfo>
 
<partinfo>BBa_K861177 short</partinfo>
  
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This part was used to produce protein FadD.
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Latest revision as of 17:44, 21 September 2012

K861162+ K861175

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 815
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1425
    Illegal AgeI site found at 1537
  • 1000
    COMPATIBLE WITH RFC[1000]