Difference between revisions of "Part:BBa K953000"

 
 
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E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS) and T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I719005">Part BBa1719005</a></html>, oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
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<i>Synechocystis</i> Heme Oxygenase with a RBS and codon optimised for expression in <i>E. coli</i>. This part differs from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html> in that it also includes a T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">Part BBa_I712074</a></html>). Heme oxygenase will oxidise heme into biliverdin and can then  complex with bacteriophytochrome (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953001">Part BBa_K953001</a></html> or <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953002">Part BBa_K953002</a></html>). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As <i>E. coli</i> does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.
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The results and characterisation of heme oxygenase can be found <html><a href="http://2012.igem.org/Team:Macquarie_Australia/Results#1">here</a></html> and you can also check out our entire project be clicking <html><a href="http://2012.igem.org/Team:Macquarie_Australia">here.</a></html>
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<center>http://25.media.tumblr.com/tumblr_mazgigGnrs1rg4kjpo1_500.jpg</center>
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<center><i>Pellets of cells containing induced heme oxygenase, uninducded heme oxygenase and lacking heme oxygenase</i></center>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:21, 27 September 2012

Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)

Synechocystis Heme Oxygenase with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953004 in that it also includes a T7 promoter (Part BBa_I712074). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome (Part BBa_K953001 or Part BBa_K953002). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.


The results and characterisation of heme oxygenase can be found here and you can also check out our entire project be clicking here.


http://25.media.tumblr.com/tumblr_mazgigGnrs1rg4kjpo1_500.jpg
Pellets of cells containing induced heme oxygenase, uninducded heme oxygenase and lacking heme oxygenase



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]