Difference between revisions of "Part:BBa K743004:Design"

(→‎Design Notes)
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===Source===
 
===Source===
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BBa_B0015 terminator sequence was obtained from the registry and ligated with Synechocystis genomic region sll0337 by Gibson assembly
 
  
 
===References===
 
===References===

Latest revision as of 00:32, 23 September 2012

psb1C3_IntK plasmid with RFP under psaAB promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2752
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2752
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2752
    Illegal XhoI site found at 3249
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2752
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2752
    Illegal AgeI site found at 1270
    Illegal AgeI site found at 1382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

Part K743001 contains a EcoR1 site in position 132. This shouldn´t represent a problem as long this composite part is digested as a downstream part (i.e with enzymes different from EcoR1) No mutation was done so that complete homology with Synechocystis chromosome is achieved

Source

References